general linear model jmp version 10.0.0 Search Results


bio rad model pds  (Bio-Rad)
96
Bio-Rad bio rad model pds
Bio Rad Model Pds, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+linear+model+jmp+version+10%2E0%2E0/PDS-1000%2FHe+System/pmc04826836-316-27-27
Average 96 stars, based on 1 article reviews
bio rad model pds - by Bioz Stars, 2026-09
96/100 stars
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statistical software v10  (STATA Corporation)
99
STATA Corporation statistical software v10
Statistical Software V10, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+linear+model+jmp+version+10%2E0%2E0/STATA+10%2E0/pmc03570333-66-10-9
Average 99 stars, based on 1 article reviews
statistical software v10 - by Bioz Stars, 2026-09
99/100 stars
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gapdh antibody  (Danaher Inc)
99
Danaher Inc gapdh antibody
Supplementation of EGF, bFGF, and IGF-1 enhanced the cellular proliferation of BMSC-derived NPCs via downregulation of miR-22-3p. (A) Representative photomicrograph of BMSCs in culture. Immunocytochemical staining indicated that BMSCs expressed CD90, CD44, fibronectin, vimentin, and nestin. Nuclei were counterstained with Sytox Blue. Images were viewed under a confocal microscope. Scale bar: 100 μm. (B) Photomicrograph of free-floating neurospheres generated in neurobasal media with and without growth factor supplementation. Scale bar: 100 μm. The size of each neurosphere was measured using ImageJ based on the longest diameter. (C) Immunofluorescence staining of NPCs with Sox2 (scale bar: 50 μm) and fibronectin (scale bar: 20 μm). (D) Proliferation analysis of NPCs under different growth factors. Cells were incubated with an MTS reagent for 4 h, and changes in proliferation were studied at different time intervals. Data are represented as the mean optical density (OD) at 540 nm. (E) Expression level of miR-22-3p in BMSC-derived NPCs relative to the control (without growth factor). (F) Relative expression of the Pten , Akt1 , and Tp53 genes in NPCs derived from different growth factor combinations compared with the control. The indicated fold-change values were normalized to the Actb and <t>Gapdh</t> controls. All experiments were repeated in three biological replicates. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
Gapdh Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+linear+model+jmp+version+10%2E0%2E0/Anti-GAPDH+antibody+-+Loading+Control/pmc09449758-497-24-28
Average 99 stars, based on 1 article reviews
gapdh antibody - by Bioz Stars, 2026-09
99/100 stars
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bootstrapped data  (STATA Corporation)
99
STATA Corporation bootstrapped data
Supplementation of EGF, bFGF, and IGF-1 enhanced the cellular proliferation of BMSC-derived NPCs via downregulation of miR-22-3p. (A) Representative photomicrograph of BMSCs in culture. Immunocytochemical staining indicated that BMSCs expressed CD90, CD44, fibronectin, vimentin, and nestin. Nuclei were counterstained with Sytox Blue. Images were viewed under a confocal microscope. Scale bar: 100 μm. (B) Photomicrograph of free-floating neurospheres generated in neurobasal media with and without growth factor supplementation. Scale bar: 100 μm. The size of each neurosphere was measured using ImageJ based on the longest diameter. (C) Immunofluorescence staining of NPCs with Sox2 (scale bar: 50 μm) and fibronectin (scale bar: 20 μm). (D) Proliferation analysis of NPCs under different growth factors. Cells were incubated with an MTS reagent for 4 h, and changes in proliferation were studied at different time intervals. Data are represented as the mean optical density (OD) at 540 nm. (E) Expression level of miR-22-3p in BMSC-derived NPCs relative to the control (without growth factor). (F) Relative expression of the Pten , Akt1 , and Tp53 genes in NPCs derived from different growth factor combinations compared with the control. The indicated fold-change values were normalized to the Actb and <t>Gapdh</t> controls. All experiments were repeated in three biological replicates. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
Bootstrapped Data, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+linear+model+jmp+version+10%2E0%2E0/STATA+12%2E0/pmc04478129-174-11-23
Average 99 stars, based on 1 article reviews
bootstrapped data - by Bioz Stars, 2026-09
99/100 stars
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digital polarimeter  (JASCO Inc)
99
JASCO Inc digital polarimeter
Supplementation of EGF, bFGF, and IGF-1 enhanced the cellular proliferation of BMSC-derived NPCs via downregulation of miR-22-3p. (A) Representative photomicrograph of BMSCs in culture. Immunocytochemical staining indicated that BMSCs expressed CD90, CD44, fibronectin, vimentin, and nestin. Nuclei were counterstained with Sytox Blue. Images were viewed under a confocal microscope. Scale bar: 100 μm. (B) Photomicrograph of free-floating neurospheres generated in neurobasal media with and without growth factor supplementation. Scale bar: 100 μm. The size of each neurosphere was measured using ImageJ based on the longest diameter. (C) Immunofluorescence staining of NPCs with Sox2 (scale bar: 50 μm) and fibronectin (scale bar: 20 μm). (D) Proliferation analysis of NPCs under different growth factors. Cells were incubated with an MTS reagent for 4 h, and changes in proliferation were studied at different time intervals. Data are represented as the mean optical density (OD) at 540 nm. (E) Expression level of miR-22-3p in BMSC-derived NPCs relative to the control (without growth factor). (F) Relative expression of the Pten , Akt1 , and Tp53 genes in NPCs derived from different growth factor combinations compared with the control. The indicated fold-change values were normalized to the Actb and <t>Gapdh</t> controls. All experiments were repeated in three biological replicates. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
Digital Polarimeter, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+linear+model+jmp+version+10%2E0%2E0/JASCO+Digital+Polarimeter/pm17409573-44-10-9
Average 99 stars, based on 1 article reviews
digital polarimeter - by Bioz Stars, 2026-09
99/100 stars
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general linear model (glm) procedure  (ncss llc)
90
ncss llc general linear model (glm) procedure
Supplementation of EGF, bFGF, and IGF-1 enhanced the cellular proliferation of BMSC-derived NPCs via downregulation of miR-22-3p. (A) Representative photomicrograph of BMSCs in culture. Immunocytochemical staining indicated that BMSCs expressed CD90, CD44, fibronectin, vimentin, and nestin. Nuclei were counterstained with Sytox Blue. Images were viewed under a confocal microscope. Scale bar: 100 μm. (B) Photomicrograph of free-floating neurospheres generated in neurobasal media with and without growth factor supplementation. Scale bar: 100 μm. The size of each neurosphere was measured using ImageJ based on the longest diameter. (C) Immunofluorescence staining of NPCs with Sox2 (scale bar: 50 μm) and fibronectin (scale bar: 20 μm). (D) Proliferation analysis of NPCs under different growth factors. Cells were incubated with an MTS reagent for 4 h, and changes in proliferation were studied at different time intervals. Data are represented as the mean optical density (OD) at 540 nm. (E) Expression level of miR-22-3p in BMSC-derived NPCs relative to the control (without growth factor). (F) Relative expression of the Pten , Akt1 , and Tp53 genes in NPCs derived from different growth factor combinations compared with the control. The indicated fold-change values were normalized to the Actb and <t>Gapdh</t> controls. All experiments were repeated in three biological replicates. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
General Linear Model (Glm) Procedure, supplied by ncss llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+linear+model+jmp+version+10%2E0%2E0/generalized+linear+model/10__1590_slash_0103___8478cr20190537-83-23-28
Average 90 stars, based on 1 article reviews
general linear model (glm) procedure - by Bioz Stars, 2026-09
90/100 stars
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micropipette puller  (Sutter Instrument Company)
96
Sutter Instrument Company micropipette puller
Supplementation of EGF, bFGF, and IGF-1 enhanced the cellular proliferation of BMSC-derived NPCs via downregulation of miR-22-3p. (A) Representative photomicrograph of BMSCs in culture. Immunocytochemical staining indicated that BMSCs expressed CD90, CD44, fibronectin, vimentin, and nestin. Nuclei were counterstained with Sytox Blue. Images were viewed under a confocal microscope. Scale bar: 100 μm. (B) Photomicrograph of free-floating neurospheres generated in neurobasal media with and without growth factor supplementation. Scale bar: 100 μm. The size of each neurosphere was measured using ImageJ based on the longest diameter. (C) Immunofluorescence staining of NPCs with Sox2 (scale bar: 50 μm) and fibronectin (scale bar: 20 μm). (D) Proliferation analysis of NPCs under different growth factors. Cells were incubated with an MTS reagent for 4 h, and changes in proliferation were studied at different time intervals. Data are represented as the mean optical density (OD) at 540 nm. (E) Expression level of miR-22-3p in BMSC-derived NPCs relative to the control (without growth factor). (F) Relative expression of the Pten , Akt1 , and Tp53 genes in NPCs derived from different growth factor combinations compared with the control. The indicated fold-change values were normalized to the Actb and <t>Gapdh</t> controls. All experiments were repeated in three biological replicates. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
Micropipette Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+linear+model+jmp+version+10%2E0%2E0/P-1000+Next+Generation+Micropipette+Puller/pmc12319296-53-16-20
Average 96 stars, based on 1 article reviews
micropipette puller - by Bioz Stars, 2026-09
96/100 stars
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pulse generator sentiva 1000  (LivaNova Inc)
90
LivaNova Inc pulse generator sentiva 1000
Supplementation of EGF, bFGF, and IGF-1 enhanced the cellular proliferation of BMSC-derived NPCs via downregulation of miR-22-3p. (A) Representative photomicrograph of BMSCs in culture. Immunocytochemical staining indicated that BMSCs expressed CD90, CD44, fibronectin, vimentin, and nestin. Nuclei were counterstained with Sytox Blue. Images were viewed under a confocal microscope. Scale bar: 100 μm. (B) Photomicrograph of free-floating neurospheres generated in neurobasal media with and without growth factor supplementation. Scale bar: 100 μm. The size of each neurosphere was measured using ImageJ based on the longest diameter. (C) Immunofluorescence staining of NPCs with Sox2 (scale bar: 50 μm) and fibronectin (scale bar: 20 μm). (D) Proliferation analysis of NPCs under different growth factors. Cells were incubated with an MTS reagent for 4 h, and changes in proliferation were studied at different time intervals. Data are represented as the mean optical density (OD) at 540 nm. (E) Expression level of miR-22-3p in BMSC-derived NPCs relative to the control (without growth factor). (F) Relative expression of the Pten , Akt1 , and Tp53 genes in NPCs derived from different growth factor combinations compared with the control. The indicated fold-change values were normalized to the Actb and <t>Gapdh</t> controls. All experiments were repeated in three biological replicates. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
Pulse Generator Sentiva 1000, supplied by LivaNova Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+linear+model+jmp+version+10%2E0%2E0/vns+therapy+system/pm39072841-81-48-50
Average 90 stars, based on 1 article reviews
pulse generator sentiva 1000 - by Bioz Stars, 2026-09
90/100 stars
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model spectrum 1000  (Revvity)
91
Revvity model spectrum 1000
Supplementation of EGF, bFGF, and IGF-1 enhanced the cellular proliferation of BMSC-derived NPCs via downregulation of miR-22-3p. (A) Representative photomicrograph of BMSCs in culture. Immunocytochemical staining indicated that BMSCs expressed CD90, CD44, fibronectin, vimentin, and nestin. Nuclei were counterstained with Sytox Blue. Images were viewed under a confocal microscope. Scale bar: 100 μm. (B) Photomicrograph of free-floating neurospheres generated in neurobasal media with and without growth factor supplementation. Scale bar: 100 μm. The size of each neurosphere was measured using ImageJ based on the longest diameter. (C) Immunofluorescence staining of NPCs with Sox2 (scale bar: 50 μm) and fibronectin (scale bar: 20 μm). (D) Proliferation analysis of NPCs under different growth factors. Cells were incubated with an MTS reagent for 4 h, and changes in proliferation were studied at different time intervals. Data are represented as the mean optical density (OD) at 540 nm. (E) Expression level of miR-22-3p in BMSC-derived NPCs relative to the control (without growth factor). (F) Relative expression of the Pten , Akt1 , and Tp53 genes in NPCs derived from different growth factor combinations compared with the control. The indicated fold-change values were normalized to the Actb and <t>Gapdh</t> controls. All experiments were repeated in three biological replicates. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
Model Spectrum 1000, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+linear+model+jmp+version+10%2E0%2E0/Caps+Poly+Liner+%2F+1000/10__3390_slash_molecules14072410-215-13-10
Average 91 stars, based on 1 article reviews
model spectrum 1000 - by Bioz Stars, 2026-09
91/100 stars
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automated syringe pump  (Pump Systems Inc)
90
Pump Systems Inc automated syringe pump
Supplementation of EGF, bFGF, and IGF-1 enhanced the cellular proliferation of BMSC-derived NPCs via downregulation of miR-22-3p. (A) Representative photomicrograph of BMSCs in culture. Immunocytochemical staining indicated that BMSCs expressed CD90, CD44, fibronectin, vimentin, and nestin. Nuclei were counterstained with Sytox Blue. Images were viewed under a confocal microscope. Scale bar: 100 μm. (B) Photomicrograph of free-floating neurospheres generated in neurobasal media with and without growth factor supplementation. Scale bar: 100 μm. The size of each neurosphere was measured using ImageJ based on the longest diameter. (C) Immunofluorescence staining of NPCs with Sox2 (scale bar: 50 μm) and fibronectin (scale bar: 20 μm). (D) Proliferation analysis of NPCs under different growth factors. Cells were incubated with an MTS reagent for 4 h, and changes in proliferation were studied at different time intervals. Data are represented as the mean optical density (OD) at 540 nm. (E) Expression level of miR-22-3p in BMSC-derived NPCs relative to the control (without growth factor). (F) Relative expression of the Pten , Akt1 , and Tp53 genes in NPCs derived from different growth factor combinations compared with the control. The indicated fold-change values were normalized to the Actb and <t>Gapdh</t> controls. All experiments were repeated in three biological replicates. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
Automated Syringe Pump, supplied by Pump Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+linear+model+jmp+version+10%2E0%2E0/automated+syringe+pump/10__1039_slash_c5cp01171j-57-9-11
Average 90 stars, based on 1 article reviews
automated syringe pump - by Bioz Stars, 2026-09
90/100 stars
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model p 1000 flaming brown micropipette puller  (Sutter Instrument Company)
96
Sutter Instrument Company model p 1000 flaming brown micropipette puller
Supplementation of EGF, bFGF, and IGF-1 enhanced the cellular proliferation of BMSC-derived NPCs via downregulation of miR-22-3p. (A) Representative photomicrograph of BMSCs in culture. Immunocytochemical staining indicated that BMSCs expressed CD90, CD44, fibronectin, vimentin, and nestin. Nuclei were counterstained with Sytox Blue. Images were viewed under a confocal microscope. Scale bar: 100 μm. (B) Photomicrograph of free-floating neurospheres generated in neurobasal media with and without growth factor supplementation. Scale bar: 100 μm. The size of each neurosphere was measured using ImageJ based on the longest diameter. (C) Immunofluorescence staining of NPCs with Sox2 (scale bar: 50 μm) and fibronectin (scale bar: 20 μm). (D) Proliferation analysis of NPCs under different growth factors. Cells were incubated with an MTS reagent for 4 h, and changes in proliferation were studied at different time intervals. Data are represented as the mean optical density (OD) at 540 nm. (E) Expression level of miR-22-3p in BMSC-derived NPCs relative to the control (without growth factor). (F) Relative expression of the Pten , Akt1 , and Tp53 genes in NPCs derived from different growth factor combinations compared with the control. The indicated fold-change values were normalized to the Actb and <t>Gapdh</t> controls. All experiments were repeated in three biological replicates. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
Model P 1000 Flaming Brown Micropipette Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+linear+model+jmp+version+10%2E0%2E0/P-1000+Next+Generation+Micropipette+Puller/pmc06218699__mmc1-63-26-31
Average 96 stars, based on 1 article reviews
model p 1000 flaming brown micropipette puller - by Bioz Stars, 2026-09
96/100 stars
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hungary semprep  (Technoorg Linda Co Ltd)
94
Technoorg Linda Co Ltd hungary semprep
Supplementation of EGF, bFGF, and IGF-1 enhanced the cellular proliferation of BMSC-derived NPCs via downregulation of miR-22-3p. (A) Representative photomicrograph of BMSCs in culture. Immunocytochemical staining indicated that BMSCs expressed CD90, CD44, fibronectin, vimentin, and nestin. Nuclei were counterstained with Sytox Blue. Images were viewed under a confocal microscope. Scale bar: 100 μm. (B) Photomicrograph of free-floating neurospheres generated in neurobasal media with and without growth factor supplementation. Scale bar: 100 μm. The size of each neurosphere was measured using ImageJ based on the longest diameter. (C) Immunofluorescence staining of NPCs with Sox2 (scale bar: 50 μm) and fibronectin (scale bar: 20 μm). (D) Proliferation analysis of NPCs under different growth factors. Cells were incubated with an MTS reagent for 4 h, and changes in proliferation were studied at different time intervals. Data are represented as the mean optical density (OD) at 540 nm. (E) Expression level of miR-22-3p in BMSC-derived NPCs relative to the control (without growth factor). (F) Relative expression of the Pten , Akt1 , and Tp53 genes in NPCs derived from different growth factor combinations compared with the control. The indicated fold-change values were normalized to the Actb and <t>Gapdh</t> controls. All experiments were repeated in three biological replicates. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
Hungary Semprep, supplied by Technoorg Linda Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/general+linear+model+jmp+version+10%2E0%2E0/SEMPREP+SMART/10__3390_slash_met12040678-117-24-22
Average 94 stars, based on 1 article reviews
hungary semprep - by Bioz Stars, 2026-09
94/100 stars
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Image Search Results


Supplementation of EGF, bFGF, and IGF-1 enhanced the cellular proliferation of BMSC-derived NPCs via downregulation of miR-22-3p. (A) Representative photomicrograph of BMSCs in culture. Immunocytochemical staining indicated that BMSCs expressed CD90, CD44, fibronectin, vimentin, and nestin. Nuclei were counterstained with Sytox Blue. Images were viewed under a confocal microscope. Scale bar: 100 μm. (B) Photomicrograph of free-floating neurospheres generated in neurobasal media with and without growth factor supplementation. Scale bar: 100 μm. The size of each neurosphere was measured using ImageJ based on the longest diameter. (C) Immunofluorescence staining of NPCs with Sox2 (scale bar: 50 μm) and fibronectin (scale bar: 20 μm). (D) Proliferation analysis of NPCs under different growth factors. Cells were incubated with an MTS reagent for 4 h, and changes in proliferation were studied at different time intervals. Data are represented as the mean optical density (OD) at 540 nm. (E) Expression level of miR-22-3p in BMSC-derived NPCs relative to the control (without growth factor). (F) Relative expression of the Pten , Akt1 , and Tp53 genes in NPCs derived from different growth factor combinations compared with the control. The indicated fold-change values were normalized to the Actb and Gapdh controls. All experiments were repeated in three biological replicates. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Journal: Heliyon

Article Title: Transplantation of IGF-1-induced BMSC-derived NPCs promotes tissue repair and motor recovery in a rat spinal cord injury model

doi: 10.1016/j.heliyon.2022.e10384

Figure Lengend Snippet: Supplementation of EGF, bFGF, and IGF-1 enhanced the cellular proliferation of BMSC-derived NPCs via downregulation of miR-22-3p. (A) Representative photomicrograph of BMSCs in culture. Immunocytochemical staining indicated that BMSCs expressed CD90, CD44, fibronectin, vimentin, and nestin. Nuclei were counterstained with Sytox Blue. Images were viewed under a confocal microscope. Scale bar: 100 μm. (B) Photomicrograph of free-floating neurospheres generated in neurobasal media with and without growth factor supplementation. Scale bar: 100 μm. The size of each neurosphere was measured using ImageJ based on the longest diameter. (C) Immunofluorescence staining of NPCs with Sox2 (scale bar: 50 μm) and fibronectin (scale bar: 20 μm). (D) Proliferation analysis of NPCs under different growth factors. Cells were incubated with an MTS reagent for 4 h, and changes in proliferation were studied at different time intervals. Data are represented as the mean optical density (OD) at 540 nm. (E) Expression level of miR-22-3p in BMSC-derived NPCs relative to the control (without growth factor). (F) Relative expression of the Pten , Akt1 , and Tp53 genes in NPCs derived from different growth factor combinations compared with the control. The indicated fold-change values were normalized to the Actb and Gapdh controls. All experiments were repeated in three biological replicates. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Article Snippet: The primary antibodies used included rabbit anti-Sox2 antibody, rabbit anti-nestin antibody, rabbit anti-beta III tubulin antibody, rabbit anti-Map2 antibody, rabbit anti-myelin basic protein antibody, GAPDH antibody (all 1:1000; Abcam, Cambridge, UK), rabbit anti-GFAP antibody (1:5000, Abcam, Cambridge, UK), and rabbit anti-APC antibody (1:50, Abcam, Cambridge, UK), followed by incubation with the secondary antibody, goat anti-rabbit IgG (HRP) (Thermo Fisher Scientific, USA).

Techniques: Derivative Assay, Staining, Microscopy, Generated, Immunofluorescence, Incubation, Expressing, Control

Transplantation of BMSC-derived NPCs in a spinal cord injury animal model promoted locomotor and sensory recoveries (N = 3 per group). (A) A 4-mm-long longitudinal cut along the midline of the spinal cord for lateral hemisection at the T9-T10 level. (B) Timeline of the entire in vivo transplantation study. (C) Open-field locomotor assessment. The hind limb function of all rats was assessed using the Basso, Beattie, and Bresnahan (BBB) locomotor scale. (D) Mechanical sensory assessment as conducted with the Von Frey filament test. Two-way ANOVA was performed, followed by a Tukey test to compare the mean difference among the groups and times. An asterisk (∗) indicates a significant difference of the treatment groups and the control group. A hashtag (#) indicates a significant difference between treatment days. (E) Relative expression of Map2, Gfap, Olig2, Mbp, Sox2 , and nestin within the transplanted region of the spinal cord. (F) Western blot analysis and relative quantification of TUJ1, GFAP, APC, and MBP. Each protein band was normalized to GAPDH. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA to compare within groups. ∗ p < 0.05, # p < 0.05, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Journal: Heliyon

Article Title: Transplantation of IGF-1-induced BMSC-derived NPCs promotes tissue repair and motor recovery in a rat spinal cord injury model

doi: 10.1016/j.heliyon.2022.e10384

Figure Lengend Snippet: Transplantation of BMSC-derived NPCs in a spinal cord injury animal model promoted locomotor and sensory recoveries (N = 3 per group). (A) A 4-mm-long longitudinal cut along the midline of the spinal cord for lateral hemisection at the T9-T10 level. (B) Timeline of the entire in vivo transplantation study. (C) Open-field locomotor assessment. The hind limb function of all rats was assessed using the Basso, Beattie, and Bresnahan (BBB) locomotor scale. (D) Mechanical sensory assessment as conducted with the Von Frey filament test. Two-way ANOVA was performed, followed by a Tukey test to compare the mean difference among the groups and times. An asterisk (∗) indicates a significant difference of the treatment groups and the control group. A hashtag (#) indicates a significant difference between treatment days. (E) Relative expression of Map2, Gfap, Olig2, Mbp, Sox2 , and nestin within the transplanted region of the spinal cord. (F) Western blot analysis and relative quantification of TUJ1, GFAP, APC, and MBP. Each protein band was normalized to GAPDH. Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA to compare within groups. ∗ p < 0.05, # p < 0.05, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Article Snippet: The primary antibodies used included rabbit anti-Sox2 antibody, rabbit anti-nestin antibody, rabbit anti-beta III tubulin antibody, rabbit anti-Map2 antibody, rabbit anti-myelin basic protein antibody, GAPDH antibody (all 1:1000; Abcam, Cambridge, UK), rabbit anti-GFAP antibody (1:5000, Abcam, Cambridge, UK), and rabbit anti-APC antibody (1:50, Abcam, Cambridge, UK), followed by incubation with the secondary antibody, goat anti-rabbit IgG (HRP) (Thermo Fisher Scientific, USA).

Techniques: Transplantation Assay, Derivative Assay, Animal Model, In Vivo, Control, Expressing, Western Blot, Quantitative Proteomics